Nicotine and Metabolites, Urine

Preferred specimen:	4.0 mL random urine with no additives or preservatives.
Minimum specimen:	1.0 mL urine
Notes:	Drugs covered: nicotine, cotinine (metabolite), 3-OH-cotinine (metabolite), nornicotine (metabolite), anabasine (tobacco biomarker). All drug and metabolites are the non-glucuronidated (free) form. Positive cutoff: Nicotine 2 ng/mL Cotinine 5 ng/mL 3-OH-Cotinine 50 ng/mL Nornicotine 2 ng/mL Anabasine 3 ng/mL The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.
Transport temp:	Room temperature
Methodology:	Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Unit Code:	828475
Ref Range:	By report
Reported:	2-5 days
	ARUP Consult® Disease Topics Nicotine & Metabolites