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| Heparin-Induced Thrombocytopenia (HIT) Antibody, PF4 IgG with Reflex to Serotonin Release Assay (Heparin Dependent Platelet Antibody), Unfractionated Heparin |
| Preferred specimen: |
5.0 mL serum, gel-barrier tube. Remove serum from cells ASAP or within 2 hours of collection, transfer to a plastic transport tube and freeze. Separate specimens must be submitted when multiple tests are ordered. |
| Minimum specimen: |
2.0 mL serum |
| Notes: |
This is the gold standard reflex testing for confirming diagnosis of HIT. Serotonin Release Assay is performed for positive HIT Antibody, IgG. If Heparin-Induced Thrombocytopenia (HIT) PF4 Antibody, IgG is 0.400 O.D. or greater, Serotonin Release Assay (Heparin Dependent Platelet Antibody), Unfractionated Heparin will be added at an additional charge. |
| Unacceptable specimen: |
Heparinized or nonfrozen specimens. Microbially contaminated, heat-inactivated, hemolyzed, icteric, or lipemic sera may give inconsistent results. |
| Transport temp: |
CRITICAL FROZEN |
| Methodology: |
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Serotoniin Release Assay |
| Unit Code: |
818345 |
| Ref Range: |
Heparin-Induced Thrombocytopenia (HIT) PF4 Antibody, IgG: Less than or equal to 0.399 OD
Serotonin Release Assay (Heparin Dependent Platelet Antibody), Unfractionated Heparin: Negative
This ELISA assay detects the presence of IgG antibodies to heparin-platelet factor 4 (PF4) complexes. Most cases of heparin-induced thrombocytopenia (HIT) are caused by IgG antibodies to heparin-PF4, rather than IgA or IgM antibodies. Negative results have a good negative predictive value for HIT, although rare false-negative results may occur. Positive ELISA results are sensitive but not completely specific for HIT. HIT is a clinicopathologic diagnosis. Clinical findings and the results of other laboratory tests must be taken into consideration. Higher optical density (OD) values in the IgG ELISA test correlate with a higher likelihood of positivity in platelet activation assays, such as the serotonin release assay (SRA), and an increased likelihood of clinical HIT. |
| Reported: |
2-7 days |
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